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小鼠角化細胞內(nèi)分泌因子酶免試劑盒,(KAF)/雙調(diào)蛋白酶免試劑盒,(AR)ELISA檢測試劑盒,英文名:AR ELISA Kit,英文縮寫:AR ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠角化細胞內(nèi)分泌因子ELISA檢測試劑盒,KAF)/雙調(diào)蛋白ELISA檢測試劑盒,AR 檢測試劑盒,AR 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠角化細胞內(nèi)分泌因子酶免試劑盒,(KAF)/雙調(diào)蛋白酶免試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復(fù)性。
彈性蛋白酶2B(ELA2B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elastase 2B (ELA2B)48T/96TY13635Homo sapiens (Human)
血管生成素1(ANGPT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin 1 (ANGPT1)48T/96TY13636Canis familiaris; Canine (Dog)
血小板反應(yīng)蛋白解整合素金屬肽酶9(ADAMTS9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 9 (ADAMTS9)48T/96TY13637Homo sapiens (Human)
肉毒堿棕櫚?;D(zhuǎn)移酶1A(CPT1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnitine Palmitoyltransferase 1A, Liver (CPT1A)48T/96TY13638Mus musculus (Mouse)
P物質(zhì)(SP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Substance P (SP)48T/96TY13639Cavia (Guinea pig )
皮質(zhì)醇(Cortisol)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cortisol48T/96TY13640General
尿調(diào)蛋白(UMOD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uromodulin (UMOD)48T/96TY13641Homo sapiens (Human)
異檸檬酸脫氫酶1(IDH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Isocitrate Dehydrogenase 1, Soluble (IDH1)48T/96TY13642Rattus norvegicus (Rat)
N-乙酰神經(jīng)氨酰酸合酶(NANS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-Acetylneuraminic Acid Synthase (NANS)48T/96TY13643Homo sapiens (Human)
4-羥基苯丙酮酸雙加氧酶(HPD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD)48T/96TY13644Homo sapiens (Human)
碘甲腺原氨酸脫碘酶Ⅲ(DIO3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Deiodinase, Iodothyronine, Type III (DIO3)48T/96TY13645Mus musculus (Mouse)
氫離子轉(zhuǎn)運ATP酶溶酶體輔助蛋白2(ATP6AP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2)48T/96TY13646Rattus norvegicus (Rat)
芳香烴受體核轉(zhuǎn)位因子(ARNT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)48T/96TY13647Mus musculus (Mouse)
白細胞衍生趨化因子2(LECT2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukocyte Cell Derived Chemotaxin 2 (LECT2)48T/96TY13648Homo sapiens (Human)
腫瘤壞死因子α誘導(dǎo)蛋白2(TNFαIP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2)48T/96TY13649Homo sapiens (Human)
環(huán)腺苷二磷酸核糖水解酶(cADPRH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclic ADP Ribose Hydrolase (cADPRH)48T/96TY13650Rattus norvegicus (Rat)
Klothoβ蛋白(KLβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Klotho Beta (KLb)48T/96TY13651Homo sapiens (Human)
脯氨酰4-羥化酶α多肽Ⅱ(P4Hα2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2)48T/96TY13652Homo sapiens (Human)
N-myc下游調(diào)節(jié)基因2(NDRG2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for N-myc Downstream Regulated Gene 2 (NDRG2)48T/96TY13653Rattus norvegicus (Rat)
骨橋素(OPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteopontin (OPN)48T/96TY13654Sus scrofa; Porcine (Pig)
70kDa熱休克蛋白8(HSPA8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 8 (HSPA8)48T/96TY13655Rattus norvegicus (Rat)
Ⅲ型膠原α1(COL3α1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type III Alpha 1 (COL3a1)48T/96TY13656Rattus norvegicus (Rat)
補體應(yīng)答基因32(RGC32)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Response Gene To Complement 32 (RGC32)48T/96TY13657Homo sapiens (Human)
角蛋白9(KRT9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Keratin 9 (KRT9)48T/96TY13658Homo sapiens (Human)
松弛肽(RLN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Relaxin (RLN)48T/96TY13659Mus musculus (Mouse)
無孢蛋白(ASPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Asporin (ASPN)48T/96TY13660Mus musculus (Mouse)
突觸融合蛋白2(STX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Syntaxin 2 (STX2)48T/96TY13661Homo sapiens (Human)
白介素22受體α2(IL2Rα2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 22 Receptor Alpha 2 (IL22Ra2)48T/96TY13662Mus musculus (Mouse)
溶質(zhì)載體家族3成員2(SLC3A2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Solute Carrier Family 3, Member 2 (SLC3A2)48T/96TY13663Homo sapiens (Human)
骨織素(OSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteocrin (OSTN)48T/96TY13664Homo sapiens (Human)
骨織素(OSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteocrin (OSTN)48T/96TY13665Rattus norvegicus (Rat)
線粒體解偶聯(lián)蛋白2(UCP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Uncoupling Protein 2, Mitochondrial (UCP2)48T/96TY13666Rattus norvegicus (Rat)
神經(jīng)上皮細胞轉(zhuǎn)化基因1(NET1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuroepithelial Cell Transforming Gene 1 (NET1)48T/96TY13667Homo sapiens (Human)
膜突蛋白()檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Moesin ()48T/96TY13668Homo sapiens (Human)
G蛋白偶聯(lián)受體家族C5組成員A(GPRC5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for G Protein Coupled Receptor, Family C, Group 5, Member A (GPRC5A)48T/96TY13669Homo sapiens (Human)
趨化因子C-X-C-基元受體6(CXCR6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X-C-Motif Receptor 6 (CXCR6)48T/96TY13670Homo sapiens (Human)
腱蛋白(CHRD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chordin (CHRD)48T/96TY13671Rattus norvegicus (Rat)
轉(zhuǎn)化受體電位陽離子通道亞家族V成員2(TRPV2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transient Receptor Potential Cation Channel Subfamily V, Member 2 (TRPV2)48T/96TY13672Mus musculus (Mouse)
亮氨酰/半胱氨酰氨肽酶(LNPEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leucyl/Cystinyl Aminopeptidase (LNPEP)48T/96TY13673Homo sapiens (Human)
脂聯(lián)素受體2(ADIPOR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adiponectin Receptor 2 (ADIPOR2)48T/96TY13674Rattus norvegicus (Rat)
親環(huán)素A(CYPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclophilin A (CYPA)48T/96TY13675Rattus norvegicus (Rat)
朊病毒蛋白(PRNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prion Protein (PRNP)48T/96TY13676Mus musculus (Mouse)
血小板反應(yīng)蛋白解整合素金屬肽酶1(ADAMTS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)48T/96TY13677Rattus norvegicus (Rat)
信號素3A(SEMA3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Semaphorin 3A (SEMA3A)48T/96TY13678Rattus norvegicus (Rat)
Polycomb組環(huán)指蛋白4(PCGF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Polycomb Group Ring Finger Protein 4 (PCGF4)48T/96TY13679Homo sapiens (Human)
三肽基肽酶Ⅰ(TPP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tripeptidyl Peptidase I (TPP1)48T/96TY13680Homo sapiens (Human)
碳酸酐酶ⅤB(CA5B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carbonic Anhydrase VB, Mitochondrial (CA5B)48T/96TY13681Homo sapiens (Human)
谷光甘肽合成酶(GSS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glutathione Synthetase (GSS)48T/96TY13682Rattus norvegicus (Rat)
肝配蛋白A3(EFNA3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ephrin A3 (EFNA3)48T/96TY13683Homo sapiens (Human)
中期因子(MK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Midkine (MK)48T/96TY13684Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。